Fig 2: Survival patterns of ARID1A, CSMD1, and SENP3 expression in HCC tissues. (A) Survival pattern of ARID1A expression. P > 0.05. (B) Survival pattern of CSMD1 gene. P > 0.05.

Fig 3: ARID1A, CSMD1, and SENP3 expression differed significantly between tumor and paracarcinoma tissues. The IHC assay images of ARID1A (A, B), CSMD1 (C, D), and SENP3 (E, F) expression. Left images are tumor tissues (A, C, E) and right images are paracarcinoma tissues (B, D, F). (G) The positive expression rates of ARID1A, CSMD1, and SENP3 in tumor and paracarcinoma tissues. (H) The staining intensity of ARID1A, CSMD1, and SENP3 in tumor and paracarcinoma tissues. (I) The staining intensity × positive expression rate of ARID1A, CSMD1, and SENP3 in tumor and paracarcinoma tissues. Immunofluorescence staining, ×200. A paired-samples t-test was performed to test the difference between tumor and paracarcinoma tissues. Data are shown as mean ± SD. **, P < 0.05.

Fig 4: Cell invasion after the suppression of genes, ARID1A, CSMD1, and SENP3 by siRNA. The images of transwell invasion assay shown in (A) were scanned, quantified, and plotted in (B–D, respectively).

Fig 5: Prognostic value of ARID1A, CSMD1, and SENP3 expression in HCC tissues. A ROC curve analysis was performed to evaluate the prognostic power of the genes. ARID1A, CSMD1, and SENP3 yielded AUC values of 0.489 (95% CI: 0.402–0.576; P > 0.05), 0.573 (95% CI: 0.488–0.658; P > 0.05), and 0.609 (95% CI: 0.526–0.692; P = 0.013), respectively. P < 0.05 indicates statistical significance.